modified pet28a expression vector Search Results


pet28a fln elp  (New England Biolabs)
99
New England Biolabs pet28a fln elp
Pet28a Fln Elp, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/NdeI/pmc07729889__nl0c04178_si_001-6-9-37
Average 99 stars, based on 1 article reviews
pet28a fln elp - by Bioz Stars, 2026-09
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pet28a vector  (Addgene inc)
94
Addgene inc pet28a vector
Pet28a Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/pET28a(%2B)%2FFaGH16A+(Plasmid+%23100910)/bio_rxiv__653287-115-7-25
Average 94 stars, based on 1 article reviews
pet28a vector - by Bioz Stars, 2026-09
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pet28a vectors  (GenScript corporation)
90
GenScript corporation pet28a vectors
Pet28a Vectors, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/pet28a/pm38431667-279-18-20
Average 90 stars, based on 1 article reviews
pet28a vectors - by Bioz Stars, 2026-09
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pet28a expression vectors  (Promega)
90
Promega pet28a expression vectors
Catalytic function and localization of three ClLCYB proteins. A, Three ClLCYB haplotypes found in watermelons with different flesh colors: ClLCYBred with Val (V) at residue 226 and Lys (K) at residue 435 in all the red-fleshed lines; ClLCYByellow with Phe (F) at residue 226 and Asn (N) at residue 435 in the cultivated lines that did not accumulate lycopene, such as cv CS and cv JLM; and ClLCYBwhite with Phe (F) at residue 226 and Lys (K) at residue 435 in wild and semiwild white-fleshed watermelons. B, Subcellular localization of three ClLCYBs. ClLCYB-mCherry (ClLCYBwhite, ClLCYByellow, and ClLCYBred) and mCherry vector (vector control) were transiently produced in watermelon fruit protoplasts incubated in the dark for 12 h. The protoplasts were observed under chromoplast autofluorescence (column 1), under bright field (column 2), under mCherry fluorescence (column 3), and in merged images (column 4) with a confocal microscope. Bars = 20 µm. C, HPLC analysis of the color complementation of a pAC-LYC bacterial line with three ClLCYB constructs. The pAC-LYC plasmid (Cunningham and Gantt, 2007) enables lycopene production and accumulation in E. coli. <t>pET28a</t> expression vectors (Promega) carrying the three ClLCYB constructs were transformed into pAC-LYC cells, with empty pET28a as a control. Carotenoid pigment composition was examined in cultures of E. coli containing the plasmid indicated above. HPLC detection was performed at 440 nm. D, Michaelis-Menten profiles for the activities of three ClLCYBs and the concentration of the substrate lycopene in 5 mm NADPH, pH 6.5, at 25°C.
Pet28a Expression Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/pet+28a/pmc07333704-158-18-21
Average 90 stars, based on 1 article reviews
pet28a expression vectors - by Bioz Stars, 2026-09
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pet-28a- pcahg98e02  (NCIMB Ltd)
90
NCIMB Ltd pet-28a- pcahg98e02
Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- <t>pcaHG98e02</t> , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .
Pet 28a Pcahg98e02, supplied by NCIMB Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/pet+28a++pcahg98e02/pmc08667622-8-0-17
Average 90 stars, based on 1 article reviews
pet-28a- pcahg98e02 - by Bioz Stars, 2026-09
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t4 dna ligase  (New England Biolabs)
99
New England Biolabs t4 dna ligase
Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- <t>pcaHG98e02</t> , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .
T4 Dna Ligase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/T4+DNA+Ligase/10__1107_slash_s1744309112038638-40-11-14
Average 99 stars, based on 1 article reviews
t4 dna ligase - by Bioz Stars, 2026-09
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pet28a expression vector  (New England Biolabs)
99
New England Biolabs pet28a expression vector
Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- <t>pcaHG98e02</t> , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .
Pet28a Expression Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/HindIII/10__1002_slash_cmtd__70098-243-14-32
Average 99 stars, based on 1 article reviews
pet28a expression vector - by Bioz Stars, 2026-09
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recombinant prokaryote expression plasmid pet28a  (Sangon Biotech)
86
Sangon Biotech recombinant prokaryote expression plasmid pet28a
Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- <t>pcaHG98e02</t> , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .
Recombinant Prokaryote Expression Plasmid Pet28a, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/cas9+plasmid+puromycin+recombinant+sgrna/pmc13154629-88-10-17
Average 86 stars, based on 1 article reviews
recombinant prokaryote expression plasmid pet28a - by Bioz Stars, 2026-09
86/100 stars
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pet 28a vector  (Addgene inc)
94
Addgene inc pet 28a vector
Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- <t>pcaHG98e02</t> , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .
Pet 28a Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/pET-28a(%2B)-PMK+(Plasmid+%23108949)/pmc06768649-355-1-8
Average 94 stars, based on 1 article reviews
pet 28a vector - by Bioz Stars, 2026-09
94/100 stars
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pet28 vector  (Addgene inc)
94
Addgene inc pet28 vector
Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- <t>pcaHG98e02</t> , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .
Pet28 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/pET28(a)-hFX-HA+(Plasmid+%2314991)/pmc12000298-331-19-25
Average 94 stars, based on 1 article reviews
pet28 vector - by Bioz Stars, 2026-09
94/100 stars
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pet28a expression vector  (Addgene inc)
94
Addgene inc pet28a expression vector
Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- <t>pcaHG98e02</t> , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .
Pet28a Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/pET-28a(%2B)-FGF2-G3+(Plasmid+%23135521)/pmc07385400-54-4-7
Average 94 stars, based on 1 article reviews
pet28a expression vector - by Bioz Stars, 2026-09
94/100 stars
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pet28a constructs  (Addgene inc)
90
Addgene inc pet28a constructs
Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- <t>pcaHG98e02</t> , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .
Pet28a Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+pet28a+expression+vector/pET-28a(%2B)-AACT+(Plasmid+%23108944)/pmc10636026-295-19-32
Average 90 stars, based on 1 article reviews
pet28a constructs - by Bioz Stars, 2026-09
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Image Search Results


Catalytic function and localization of three ClLCYB proteins. A, Three ClLCYB haplotypes found in watermelons with different flesh colors: ClLCYBred with Val (V) at residue 226 and Lys (K) at residue 435 in all the red-fleshed lines; ClLCYByellow with Phe (F) at residue 226 and Asn (N) at residue 435 in the cultivated lines that did not accumulate lycopene, such as cv CS and cv JLM; and ClLCYBwhite with Phe (F) at residue 226 and Lys (K) at residue 435 in wild and semiwild white-fleshed watermelons. B, Subcellular localization of three ClLCYBs. ClLCYB-mCherry (ClLCYBwhite, ClLCYByellow, and ClLCYBred) and mCherry vector (vector control) were transiently produced in watermelon fruit protoplasts incubated in the dark for 12 h. The protoplasts were observed under chromoplast autofluorescence (column 1), under bright field (column 2), under mCherry fluorescence (column 3), and in merged images (column 4) with a confocal microscope. Bars = 20 µm. C, HPLC analysis of the color complementation of a pAC-LYC bacterial line with three ClLCYB constructs. The pAC-LYC plasmid (Cunningham and Gantt, 2007) enables lycopene production and accumulation in E. coli. pET28a expression vectors (Promega) carrying the three ClLCYB constructs were transformed into pAC-LYC cells, with empty pET28a as a control. Carotenoid pigment composition was examined in cultures of E. coli containing the plasmid indicated above. HPLC detection was performed at 440 nm. D, Michaelis-Menten profiles for the activities of three ClLCYBs and the concentration of the substrate lycopene in 5 mm NADPH, pH 6.5, at 25°C.

Journal: Plant Physiology

Article Title: Decreased Protein Abundance of Lycopene β -Cyclase Contributes to Red Flesh in Domesticated Watermelon 1

doi: 10.1104/pp.19.01409

Figure Lengend Snippet: Catalytic function and localization of three ClLCYB proteins. A, Three ClLCYB haplotypes found in watermelons with different flesh colors: ClLCYBred with Val (V) at residue 226 and Lys (K) at residue 435 in all the red-fleshed lines; ClLCYByellow with Phe (F) at residue 226 and Asn (N) at residue 435 in the cultivated lines that did not accumulate lycopene, such as cv CS and cv JLM; and ClLCYBwhite with Phe (F) at residue 226 and Lys (K) at residue 435 in wild and semiwild white-fleshed watermelons. B, Subcellular localization of three ClLCYBs. ClLCYB-mCherry (ClLCYBwhite, ClLCYByellow, and ClLCYBred) and mCherry vector (vector control) were transiently produced in watermelon fruit protoplasts incubated in the dark for 12 h. The protoplasts were observed under chromoplast autofluorescence (column 1), under bright field (column 2), under mCherry fluorescence (column 3), and in merged images (column 4) with a confocal microscope. Bars = 20 µm. C, HPLC analysis of the color complementation of a pAC-LYC bacterial line with three ClLCYB constructs. The pAC-LYC plasmid (Cunningham and Gantt, 2007) enables lycopene production and accumulation in E. coli. pET28a expression vectors (Promega) carrying the three ClLCYB constructs were transformed into pAC-LYC cells, with empty pET28a as a control. Carotenoid pigment composition was examined in cultures of E. coli containing the plasmid indicated above. HPLC detection was performed at 440 nm. D, Michaelis-Menten profiles for the activities of three ClLCYBs and the concentration of the substrate lycopene in 5 mm NADPH, pH 6.5, at 25°C.

Article Snippet: The pAC-LYC plasmid ( Cunningham and Gantt, 2007 ) enables lycopene production and accumulation in E. coli . pET28a expression vectors (Promega) carrying the three ClLCYB constructs were transformed into pAC-LYC cells, with empty pET28a as a control.

Techniques: Residue, Plasmid Preparation, Control, Produced, Incubation, Fluorescence, Microscopy, Construct, Expressing, Transformation Assay, Concentration Assay

Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e02 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .

Journal: Frontiers in Microbiology

Article Title: The Protocatechuate 3,4-Dioxygenase Solubility (PCDS) Tag Enhances the Expression and Solubility of Heterogenous Proteins in Escherichia coli

doi: 10.3389/fmicb.2021.779541

Figure Lengend Snippet: Determination of the expression of recombinant PcaHG in E . coli BL21 (DE3) by SDS-PAGE. Total cell extract and soluble cell extract from induced E . coli BL21 (DE3). Lane M, protein molecular mass standards (Unstained Protein Molecular Weight Marker, No. 26610, Thermo Fisher Scientific, Waltham, MA, United States). (A) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a- pcaHG98e01 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e01 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e02 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e02 . (B) lane 1, total BL21 (DE3)/pET-28a (+), lane 2, soluble BL21 (DE3)/pET-28a (+), lane 3, total BL21 (DE3)/pET-28a - pcaHG98e03 , lane 4, soluble BL21 (DE3)/pET-28a- pcaHG98e03 , lane 5, total BL21 (DE3)/pET-28a- pcaHG98e04 , lane 6, soluble BL21 (DE3)/pET-28a- pcaHG98e04 . (C) lane 1, soluble BL21 (DE3)/pET-28a- pcaHG98e04 ; lane 2. Purification of His 6 -PcaHG98e04-PCDS protein from BL21 (DE3)/pET-28a- pcaHG98e04 .

Article Snippet: pET-28a- pcaHG98e02 , Expression vector for pcaHG , PCR amplified with primers pcahg9801 and pcahg9802 from strain NCIMB 9866 genome, inserted into the Nde I- Hin d III restriction sites of pET-28a (+) , This study.

Techniques: Expressing, Recombinant, SDS Page, Molecular Weight, Marker, Purification

Determination of pcaHG98 -encoded PCA 3,4 dioxygenase activity. Spectrophotometric changes during oxidation of PCA by pcaHG98 -encoded PCA 3,4 dioxygenase. The assay of PCA catalyzed by cell extracts from E . coli BL21 (DE3) carrying pET-28a- pcaHG98e01 (A) or pET-28a- pcaHG98e04 (D) . There was no enzyme activity in the cell extracts from BL21 (DE3) carrying pET-28a- pcaHG98e02 (B) or pET-28a- pcaHG98e03 (C) .

Journal: Frontiers in Microbiology

Article Title: The Protocatechuate 3,4-Dioxygenase Solubility (PCDS) Tag Enhances the Expression and Solubility of Heterogenous Proteins in Escherichia coli

doi: 10.3389/fmicb.2021.779541

Figure Lengend Snippet: Determination of pcaHG98 -encoded PCA 3,4 dioxygenase activity. Spectrophotometric changes during oxidation of PCA by pcaHG98 -encoded PCA 3,4 dioxygenase. The assay of PCA catalyzed by cell extracts from E . coli BL21 (DE3) carrying pET-28a- pcaHG98e01 (A) or pET-28a- pcaHG98e04 (D) . There was no enzyme activity in the cell extracts from BL21 (DE3) carrying pET-28a- pcaHG98e02 (B) or pET-28a- pcaHG98e03 (C) .

Article Snippet: pET-28a- pcaHG98e02 , Expression vector for pcaHG , PCR amplified with primers pcahg9801 and pcahg9802 from strain NCIMB 9866 genome, inserted into the Nde I- Hin d III restriction sites of pET-28a (+) , This study.

Techniques: Activity Assay